WebThe lipophilicity is a crucial physicochemical parameter of every molecule, affecting toxicity, absorption, distribution, metabolism, and drug elimination [].Retention in reversed-phase high-performance liquid chromatography (RP-HPLC) is governed by lipophilicity; consequently, retention data can be considered a surrogate of log P.The International … WebFeb 15, 2003 · A procedure is described for measuring pKa values in a short time, e.g., 4 min/assay. Samples, as 10 mM solutions, are prepared in DMSO in 96-well plates. ... High-throughput measurement of pKa values in a mixed-buffer linear pH gradient system Anal Chem. 2003 Feb 15;75(4):883-92. doi: 10.1021/ac020329y.
Determining Partition Coefficient (Log P), Distribution Coefficient ...
WebApr 14, 2024 · Clean supernatant was transferred to a clean tube, diluted 1:25 in PBS for fluorescence measurement, then stored at −80 °C. For c-Peptide ELISA measurement, urine samples were measured at a final dilution of 1:50000 in PBS. For LFA measurement, urine samples were measured at a final dilution of 1:40 in PBS, 0.05% Tween-20, and 1% BSA. WebFeb 15, 2003 · The pKa values of 71 drugs have been measured, and results compare well with values measured by pH-metric or traditional UV methods. Rules are suggested for the … shared ownership huyton
High-throughput Measurement of log D and pKa - Big Chemical …
WebApr 10, 2024 · Subsequent high-throughput single-cell sequencing enables integrated analysis of the single-cell transcriptome, secretion and phenotypes (Fig. 1e, f). TRAPS-seq enables multimodal single-cell ... WebDec 22, 2006 · A high-throughput method has been developed for lipophilicity measurement. ... The composition is 2:1 (w/w) dioctyl sebacate and PVC. With six repeats, log P pw values of 15 solutes have been determined in one 96-well microplate in 4 h. A linear relationship between log ... High‐Throughput Measurement of Physicochemical … WebJun 1, 2003 · Two high-throughput assays, Calcein-AM and ATPase, identify Pgp inhibitors and modulators, which may or may not be transport substrates. A pharmacophore model … pool tables for kids walmart