WebSep 15, 2024 · The cell lysate concentration was adjusted to 1 µg/µl. Then, we added the YAP (CST, Rabbit, Cat. No. 14074S), Smad2 (CST, Rabbit, Cat. No. 5339S), and Smad3 (CST, Rabbit, Cat. No. 9523S) primary antibodies to the cell lysate individually. And an equal amount of isotype control (CST, Cat. No. 2729S) was used as a negative control. WebApr 25, 2024 · TGF-β1 binds to its receptor to trigger intracellular signaling and phosphorylates Smad2 and Smad3. Phosphorylated Smad2 and Smad3 are transported to the nucleus and regulate the transcription of target genes . Consequently, blocking the EMT of alveolar epithelial cells (AECs) might be a promising strategy for the treatment of PF.
Fibroblast-specific TGF-β-Smad2/3 signaling underlies cardiac …
Web(8828) Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb Supplier Cell Signaling Technology. Host Rabbit Type Primary Clonality Recombinant (D27F11A3) Recombinant Yes Conjugate Unconjugated Target ... CST, Cell Signalling, New England Biolabs. View additional product information. Experiments from the literature. WebHighly specific and rigorously validated in-house, Phospho-Smad2 (Ser465/467) (138D4) Rabbit Monoclonal Antibody (CST #3108) is ready to ship. ... Smad2 and Smad3 become phosphorylated at their carboxy-termini (Ser465/467 on Smad2; Ser423/425 on Smad3) by the receptor kinase TGF-β R1 (9-11). Following phosphorylation, Smad2 and Smad3 … simplify 7/40
松果菊苷对db/db糖尿病小鼠心肌的保护作用_参考网
WebJan 25, 2024 · A pan acetylation antibody (9441S, CST) was used. H) KAT6A knockdown (KD) decreases the acetylation (Ac-Lys) of SMAD3 in MDA-MB-231 cells. I) Effect of acetyltransferase activity-deficient mutants G657E and C543G/G657E of KAT6A on SMAD3 acetylation. J) MS analysis of acetylation sites of SMAD3 by KAT6A. ... In addition, in … WebSep 1, 2024 · TGF-β1 causes cardiac fibrosis by stimulating Smad3 and PI3K-Akt-GSK-3β signaling pathways. TGF-β1 phosphorylates and activates the highly-conserved cytoplasmic proteins of the SMADs family, and PI3K-Akt-GSK-3β pathway, thereby regulating the expression of the related genes leading to fibrosis. WebGet better batch-to-batch reproducibility with a recombinant antibody. Anti-Smad3 antibody [EP568Y] (ab40854) Research with confidence – consistent and reproducible results with every batch. Long-term and scalable supply – powered by recombinant technology for fast production. Success from the first experiment – confirmed specificity ... simplify . 74 24 495 1 225